Feb 8, 2024 · Here we present (F)luorescence (L)ifetime (U)l (t)imate (E)xplorer (FLUTE), the first open-source graphical user interface (GUI) for phasor analysis of FLIM data programmed in Python, one of the most widely used programming languages.

Nov 6, 2023 · To date, no open-source graphical user interface (GUI) for phasor analysis of FLIM data is available in Python, thus limiting the widespread use of phasor analysis in biomedical research. Here, we present Fluorescence Lifetime Ultimate Explorer (FLUTE), a Python GUI that is designed to fill this gap.

Fluorescence Lifetime Ultimate Explorer (FLUTE) provides a graphical user interface to explore Fluorescence Lifetime Microscopy (FLIM) using phasor analysis. The GUI allows for quick and interactive analysis of experimental FLIM data, and can export results for further processing.

Here, we introduce (F)luorescence (L)ifetime (U)l(t)imate (E)xplorer (FLUTE) that simplifies phasor FLIM image processing, accelerates the whole FLIM analysis and simplifies the visualization...

FLUTE is programmed in Python using PyQt5 and using NumPy and SciPy libraries. The Graphical user Interface is designed to be user-friendly and to perform different functionalities such as import...

Fluorescence Lifetime Ultimate Explorer (FLUTE) is a GUI for exploring and analyzing Fluorescence Lifetime Microscopy (FLIM) data - FLUTE/main.py at main · LaboratoryOpticsBiosciences/FLUTE

I would like to create an h264 or divx movie from frames that I generate in a python script in matplotlib. There are about 100k frames in this movie. In examples on the web [eg. 1], I have only se...

Dec 4, 2016 · Just sharing a series of posts I wrote recently about how Python is used in the film industry. The post linked outlines the pipeline in general, and there are individual case study posts for films I've worked on.

Oct 18, 2020 · Here you can see the various stages of a visual effects film or animated feature. Studios may differ slightly from this, but it’s the general workflow. The storyboards/footage/previs represent the data we get, and Compositing/Lighting are the last stages of the film for us.

Apr 2, 2023 · FLUTE simplifies and automates many aspects of FLIM analysis, such as calibrating the FLIM data, performing interactive exploration of the phasor plot with cursors, displaying the phasor plot and the FLIM images with different lifetime contrasts and calculating the relative concentration of molecular species.