Here we outline the application of the Azoxymethane (AOM)/Dextran sodium sulfate (DSS) model of CAC. The AOM/DSS model is a powerful, reproducible, and relatively inexpensive initiation-promotion model that utilizes chemical induction of DNA damage followed by …

Here we describe the Azoxymethane (AOM)/Dextran Sodium Sulfate (DSS) mouse model of inflammatory colorectal cancer. Keywords: AOM; Colitis-associated cancer; Colon cancer; DSS; Inflammatory carcinogenesis.

AOM/DSS模型 是目前使用最广泛的 UC化学诱导模型，临床症状学、形态学及病理学观察证明，该模型与人类 溃疡性结肠炎 癌变过程极为相似。 AOM引起 鸟嘌呤O6甲基化 是诱导 基因突变 损伤最主要的原因，基因突变使细胞过度增殖，形成特异性的大肠肿瘤。 DSS的致炎机制尚未阐明，可能与DSS的负电荷影响 DNA 合成、抑制上皮细胞增生、破坏肠黏膜屏障、导致巨噬细胞功能障碍及肠道菌群失调有关。 图1.来源pixabay. 作用机制. 偶氮甲烷 （AOM）能诱导DNA产 …

Mar 24, 2011 · Among the chemically induced CRC models, the combination of a single hit of azoxymethane (AOM) with 1 week exposure to the inflammatory agent dextran sodium sulphate (DSS) in rodents has proven to dramatically shorten the latency time for induction of CRC and to rapidly recapitulate the aberrant crypt foci-adenoma-carcinoma sequence that occurs ...

Here we outline the application of the Azoxymethane (AOM)/Dextran sodium sulfate (DSS) model of CAC. The AOM/DSS model is a powerful, reproducible, and relatively inexpensive initiation-promotion model that utilizes chemical induction of DNA damage followed by …

The combination of DSS with AOM as a model for colitis associated cancer has gained popularity for its reproducibility, potency, low price, and ease of use. Though they have a shared mechanism, AOM has been found to be more potent and stable in solution than DMH.

Nov 12, 2019 · In this article we optimized the protocol of the AOM-DSS modeling using C57BL/6 mice for study on sporadic CRC by intraperitoneal injecting AOM solution at a proper concentration with a 100 μl sterile syringe once, feeding with DSS solution for 7 days in a roll and change DSS solution every day.

Jul 27, 2021 · In this study, we evaluated an AOM/DSS model involving a single injection of AOM in combination with three treatment cycles of a reduced (1%) dose of DSS for induction of carcinogenesis in colons of A/J and B6 mice, reportedly representing two extremes with respect to AOM susceptibility.

Oct 1, 2011 · In this article we highlight the histopathological and molecular features and describe the principal genetic and epigenetic alterations and inflammatory pathways involved in carcinogenesis in AOM/DSS-treated mice; we also present a general overview of recent experimental applications and preclinical testing of novel therapeutics in the AOM/DSS ...

Jun 1, 2016 · Here we outline the application of the Azoxymethane (AOM)/Dextran sodium sulfate (DSS) model of CAC. The AOM/DSS model is a powerful, reproducible, and relatively inexpensive initiation-promotion model that utilizes chemical induction of DNA damage followed by repeated cycles of colitis [12–15].