Isolated gel band or purified protein is digested with suitable protease after reduction/alkylation. Peptide MS and MS/MS analysis with MALDI-QIT-TOF and Mascot database search. Protein Identification ...

Electronic concepts of oxidation and reduction, redox reactions ... Friedel-Craft's alkylation and acylation, directive influence of the functional group in mono- substituted benzene.

Samples were prepared using the SP3 protocol 87 with reduction, alkylation, and trypsin digestion. Peptides were labeled with TMT6plex (ThermoFisher) and fractionated by high pH reverse phase ...

Protein was extracted and estimated using BCA (Bicinchoninic Acid) protein assay (Pierce, ThermoFisher Scientific). Reduction, alkylation, and precipitation of an equal amount of protein from each ...

reduction with 10 mmol/L dithiothreitol, and alkylation with 50 mmol/L iodoacetamide. Following acetone precipitation and drying, proteins were resuspended in 100 mmol/L triethylammonium bicarbonate ...

The protocol involves extraction of proteins from cells or tissues followed by reduction, alkylation, and digestion. Samples from each biological condition were labeled with 1 of the 6 isobaric tags ...

Headed by the new CEO Murray Auchincloss, the layoff is expected to bring about better efficiency by streamlining operations and restore the confidence of investors after series of financial ...