CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

Biological mechanisms for nuclease-based gene editing technologies ... Both ZFN and TALEN systems locate similar FokI endonucleases (the dark blue combined sphere) by DNA-binding domains on the left ...

Now, new preclinical research presents the discovery and engineering of NanoCas—an ultracompact CRISPR nuclease capable of extending CRISPR’s reach in vivo beyond liver targets. NanoCas is the ...

TALENs consist of a DNA-binding domain composed by modular TALE repeats, fused with a FokI nuclease domain. Each TALE repeat is composed by 33–35 amino acids and recognizes a single nucleotide; ...