2FAST2Q is ideal for CRISPRi-Seq, and for extracting and counting any kind of information from reads in the fastq format, such as barcodes in Bar-seq experiments. 2FAST2Q can work with sequence ...
"So, we instead looked toward how MYC is actually made." Kovalski used a method called CRISPRi to hunt for factors that influence how much MYC was produced in cancer cells. Surprisingly ...
"So, we instead looked toward how MYC is actually made." Kovalski used a method called CRISPRi to hunt for factors that influence how much MYC was produced in cancer cells. Surprisingly ...
“So, we instead looked toward how MYC is actually made.” Kovalski used a method called CRISPRi to hunt for factors that influence how much MYC was produced in cancer cells. Surprisingly, the ...
Epigenomics is the systematic analysis of the global state of gene expression not attributable to mutational changes in the underlying DNA genome. An organism has multiple, cell type-specific ...
CRISPRi and CRISPRa systems are derived from the CRISPR KO system. In these systems, the HNH and RuvC domains of Cas9 are deactivated, resulting in dCas9, which loses its cutting ability. dCas9 forms ...
These studies provide new insights into the genetic manipulation of mycoplasmas. Notably, the CRISPRi system is more appropriate than knock-down/out methods to analyze the functions of essential genes ...
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