Methods described in this paper are confined to in vitro dedifferentiated plant cell suspension cultures, which are convenient for the large-scale production of fine chemicals in bioreactors and ...

FISH-Flow, a protocol for the concurrent detection of mRNA and protein in single cells using fluorescence in situ hybridization and flow cytometry. Nature Protocols , 2017; 12 (6): 1245 DOI: 10. ...

FISH-Flow is a flow-cytometry-based protocol enabling simultaneous mRNA and protein measurements in single nonadherent mammalian cells. The authors provide both a manual and a semiautomated ...

This lack of practicality has led to the development of numerous flow cytometry protocols to try to identify these cell types (CEPs and CECs) in circulation. 7,8 Until now, the ECFC remained undefined ...