Figure 1: 13-parameter, 11-color PFC accurately defines naive T cells. Peripheral blood mononuclear cells from one donor were stained with one 11-color staining combination (Table 1) and analyzed ...

Hello @sk93mo, It seems that when these parameters are set in opposition, they convey the same concept Yes and I would argue it doesn't make sense to set them together (e.g. --buffered=0 --direct=0) ...