When 454 sequencing is used, products from individual splinkerette-PCR reactions are barcoded (BC) using a short nucleotide 'tag' sequence, and the second-round PCR primers contain sequences for ...

Three of the machines currently on the market—454, Illumina, and ABI's SOLiD—use PCR to create small groups of identical molecules, and then perform sequencing on the entire population at once.

PCR amplification also plays a key role in next-generation sequencing (NGS) techniques, amplifying DNA samples before the PCR product is used for sequencing. Specific DNA sequences can also be ...

PCR for cloning amplifies DNA fragments for ligation into a vector, either via a single base overhang or blunt end ligation. This method allows cloning with low amounts of DNA and for high throughput ...

PCR-related problems have included uneven amplification, causing overrepresentation of some sequence species and nucleotide misincorporation. In particular, sequencing genomes or genomic regions ...

PCR and sequencing. The polymerase chain reaction (PCR) and sequencing are intricately linked and vital to many fields in the life sciences. First invented in 1983, PCR is used to dramatically amplify ...

PCR-free library preparation is recognized as the gold standard for whole genome sequencing (WGS), showing more uniform coverage and improved performance in calling many different variant types ...

Pretreatment with sodium bisulfite is the basis of many methylation detection and analysis techniques. 1 Following bisulfite conversion, researchers determine the methylation status in loci of ...

PCR-free library preparation is recognized as the gold standard for whole genome sequencing (WGS), showing more uniform coverage and improved performance in calling many different variant types ...