When 454 sequencing is used, products from individual splinkerette-PCR reactions are barcoded (BC) using a short nucleotide 'tag' sequence, and the second-round PCR primers contain sequences for ...

PCR-related problems have included uneven amplification, causing overrepresentation of some sequence species and nucleotide misincorporation. In particular, sequencing genomes or genomic regions ...

Three of the machines currently on the market—454, Illumina, and ABI's SOLiD—use PCR to create small groups of identical molecules, and then perform sequencing on the entire population at once.

She later says that she intends to “sequence PCR” from people who consent in Ireland and the UK, and will challenge COVID-19 restrictions by taking "an injunction in Ireland to the high court ...

PCR for cloning amplifies DNA fragments for ligation into a vector, either via a single base overhang or blunt end ligation. This method allows cloning with low amounts of DNA and for high throughput ...

The presence of uracil instead of thymine does not affect the electrophoretic mobility or sequencing of the PCR products, and the activity UDG is destroyed during hot-start activation.

Eurofins Expands Its Global Sequencing and Mutation-Specific PCR Capabilities to Detect COVID-19 Variants of Concern and of Interest. May 25, 2021 01:20 AM Eastern Daylight Time.

The hunt for diagnostic methods that improve on RT-PCR for the detection of the SARS-CoV-2 virus exemplifies the fact that PCR techniques still come with limitations. For SARS-CoV-2 detection, false ...