This buffer should have a pH of 9.2 ± 0.5 with the ability to stay within one pH unit of this target when strong acid or base is added. Scenario 4: Prepare a buffer for an antiviral agent which is ...

1| Combine the following components of the master mix on ice and mix thoroughly: 10× KLA reaction buffer (pH 9.2): 500 mM Tris base, 160 mM (NH 4) 2 SO 4, 25 mM MgCl 2, 1% Tween 20. Note ...