Six years ago, deep in the threads of Reddit’s r/skincareaddiction, I learned about urea. I needed a moisturizer for my dry, eczema-prone skin, but my current lotion was exasperating my KP ...
After IEF in the presence of urea and a nonionic detergent, the IEF gel is equilibrated in sodium dodecyl sulfate (SDS) to prepare the proteins for SDS-PAGE. The method described here 1 uses ...
1. Prepare a polyacrylamide minigel of the desired concentration containing 8 M urea (for example, Bio-Rad Mini-Protean). 2. While the gel is polymerizing, mix the following reagents: Incubate the ...
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