Finally, dUTP can be incorporated into amplification products during PCR to make ... RT-PCR, the RT and PCR steps are performed sequentially in the same tube using the entire amount of cDNA ...

This method allows scientists to make billions ... This cDNA works as a template for the PCR amplification as in Figure 1B. This modified method is named as Reverse Transcription (RT)- PCR or ...

Thus, real-time PCR may be used as a 'low-density array'. Although PCR will never generate the throughput that is achieved by cDNA microarrays ... Another example is to make low-density arrays ...

The iScript Advanced cDNA synthesis kit for RT-qPCR offers excellent sensitivity and efficiency in both real-time PCR (using SYBR Green, EvaGreen, or probes) and conventional RT-PCR. The iScript ...