The landscape of metagenomics and microbiome research is evolving at breakneck speed. As sequencing technologies improve and ...

PCR kits and reagents are designed to provide the necessary components for the DNA polymerase reaction, such as primers, dNTPs, buffer, and enzyme. Depending on your target sequence, template ...

The alternative approach for obtaining the sequence of insertion sites is to employ 454 parallel sequencing of pools of barcoded splinkerette-PCR reactions from multiple independent tumors ().As ...

Three of the machines currently on the market—454, Illumina, and ABI's SOLiD—use PCR to create small groups of identical molecules, and then perform sequencing on the entire population at once.

PCR amplification also plays a key role in next-generation sequencing (NGS) techniques, amplifying DNA samples before the PCR product is used for sequencing. Specific DNA sequences can also be ...

PCR-related problems have included uneven amplification, causing overrepresentation of some sequence species and nucleotide misincorporation. In particular, sequencing genomes or genomic regions ...

PCR and sequencing. The polymerase chain reaction (PCR) and sequencing are intricately linked and vital to many fields in the life sciences. First invented in 1983, PCR is used to dramatically amplify ...

Culture results were in agreement to DNA sequencing in 90.5% (277) of 306 samples tested, including 263 PCR and culture negative samples and 29 culture and PCR positive samples. The sensitivity of the ...