CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

Base and Prime Editing Offer Precision CRISPR-based systems that rely on traditional Cas nucleases like Cas9 cut through both strands of DNA which can lead to undesirable and even dangerous edits to ...

For example, the commonly used Cas9 is about 1,400 amino acid residues long. “It's like a new class of tools that can be used for genome editing, not just as a principle,” said Karvelis, who was not ...