Pellet 106 cells by centrifugation (250 - 300 x g) and carefully remove supernatant Make up 1X of fixation buffer by adding 5 ml of Cytofix (BD554655) to 10 ml of DPBS Add either 300μl (for microwell ...

I have gotten similar recovery of telomerase if a do this step for 2 hours or overnight. Pellet the beads at 3500 rpm for 1 min in a table-top centrifuge (Eppendorf centrifuge model 5424 or 5415D.

6 | Collect the precipitated RNA by centrifugation at 10,000g at 4 °C for 30 min. 7 | Carefully decant the isopropanol and dissolve the RNA pellet in 0.3 ml of solution D for every 1 ml of this ...